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ModiQuest Research has developed a sensitive Antibody Based Assay to test for Peptidyl Arginine Deiminase (PAD) activity (ABAP). The ABAP is an Enzyme Linked-Immuno-Sorbent Assay (ELISA) for the determination of PAD enzyme activity in cell and tissue lysates.
The ABAP kit consists of: 1) 96-well plate coated with Arginine containing peptides 2) A ModiQuest Research mouse PAD enzyme for activity calibration 3) a proprietary ModiQuest Research monoclonal antibody specifically binding deiminated arginines. Compared to currently used assays for PAD activity determination such as the DAMO BAEE conversion test the MQR-ABAP assay is more robust, and at least 500 times more sensitive.
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