For difficult targets such as protein modification-specific monoclonal antibodies, our B-cell selection technology will enrich B-cells for the target antibody. This way, we can improve the likelihood of obtaining a specific, high affinity antibody, even for poorly immunogenic targets or difficult epitopes.
B-cell Selection Prior to Hybridoma Generation
During mouse hybridoma preparation, we can isolate the B-cells from freshly extracted lymphocytes and select for antigen-specific antibody production prior to electrofusion and hybridoma generation. This improves the chances of generating an antibody with the desired characteristics.
B-cell Selection Prior to Phage Display Antibody Library Generation
To improve the outcome of recombinant monoclonal antibody generation, we can use a B-cell selection step prior to the phage display library preparation. Following the immunization step in the chosen animal species, we isolate the lymphocytes, and screen for the antigen-specific antibody-producing B cells with a target antigen ELISA. We can use the highly enriched B-cell pools to develop phage display libraries that are consequently enriched for the antigen-specific antibody of interest.
Alternatively, we can choose up to 5 of the best individual B-cell colonies and clone the antigen-specific antibody of interest directly into our recombinant antibody expression vectors.
If you have a difficult or poorly immunogenic target, talk to us to see how we could efficiently generate a monoclonal antibody for you.
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